Publications

Recent publications (2024)

Witt, H., Harju, J., Chameau, E.M.J., Bruinsma, C.M.A., Clement, T.V.M., Nielsen, C.F., Hickson, I.D., Peterman, E.J.G., Broedersz, C-P. and Wuite, G.J.L. (2024) Ion-mediated condensation controls the mechanics of mitotic chromosomes. Nature Materials 23, 1556-1562
Liu J.C.Y. et al., (2024) Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation. Nature Struct. & Molec. Biol. 31, 1355-1367.
D'Souza J, Hickson ID. Replication fork barriers to study site-specific DNA replication perturbation. DNA Repair (Amst). 2024 Sep;141:103735.
Halliwell JA, Martin-Gonzalez J, Hashim A, Dahl JA, Hoffmann ER, Lerdrup M. Sex-specific DNA-replication in the early mammalian embryo. Nat Commun. 2024 Jul 27;15(1):6323.
von Rohden E, Jensen CFS, Andersen CY, Sønksen J, Fedder J, Thorup J, Ohl DA, Fode M, Hoffmann ER, Mamsen LS. Male fertility restoration: in vivo and in vitro stem cell-based strategies using cryopreserved testis tissue: a scoping review. Fertil Steril. 2024 Jul 9:S0015-0282(24)00603-4.
Martin CA, Sarlós K, Logan CV, Thakur RS, Parry DA, Bizard AH, Leitch A, Cleal L, Ali NS, Al-Owain MA, Allen W, Altmüller J, Aza-Carmona M, Barakat BAY, Barraza-García J, Begtrup A, Bogliolo M, Cho MT, Cruz-Rojo J, Mundi Dhahrabi HA, Elcioglu NH, GOSgene, Gorman GS, Jobling R, Kesterton I, Kishita Y, Kohda M, Le Quesne Stabej P, Malallah AJ, Nürnberg P, Ohtake A, Okazaki Y, Pujol R, Ramirez MJ, Revah-Politi A, Shimura M, Stevens P, Taylor RW, Turner L, Williams H, Wilson C, Yigit G, Zahavich L, Alkuraya FS, Surralles J, Iglesias A, Murayama K, Wollnik B, Dattani M, Heath KE, Hickson ID, Jackson AP. Mutations in TOP3A Cause a Bloom Syndrome-like Disorder. Am J Hum Genet. 2024 May 2;111(5):996
Castejon-Grinan, M., Albers, E., Simon-Carrasco, L., Aguilera, P., Sbroggio, M., Pladevall-Morera, D., Ingham, A., Lim, E., Guillen-Benitez, A., Pietrini, E., Lisby, M., Hickson, I.D. and Lopez-Contreras, A.J. (2024) PICH deficiency limits the progression of MYC-induced B-cell lymphoma. Blood Cancer Journal 14: 16.
Steinthorsdottir V, Halldorsson BV, Jonsson H, Palsson G, Oddsson A, Westergaard D, Arnadottir GA, Stefansdottir L, Banasik K, Esplin MS, Hansen TF, Brunak S, Nyegaard M, Ostrowski SR, Pedersen OBV, Erikstrup C; DBDS genomics consortium; Thorleifsson G, Nadauld LD, Haraldsson A, Steingrimsdottir T, Tryggvadottir L, Jonsdottir I, Gudbjartsson DF, Hoffmann ER, Sulem P, Holm H, Nielsen HS, Stefansson K. Variant in the synaptonemal complex protein SYCE2 associates with pregnancy loss through effect on recombination. Nat Struct Mol Biol. 2024 Apr;31(4):710-716.
Asserhøj LL, Mizrak I, Lebech Kjaer AS, Clausen TD, Hoffmann ER, Greisen G, Main KM, Madsen PL, Pinborg A, Jensen RB. Blood pressure and lipid profiles in children born after ART with frozen embryo transfer. Hum Reprod Open. 2024 Mar 20;2024(2):hoae016.
Jensen CFS, Mamsen LS, Wang D, Fode M, Giwercman A, Jørgensen N, Ohl DA, Fedder J, Hoffmann ER, Yding Andersen C, Sønksen J. Results from the first autologous grafting of adult human testis tissue: a case report. Hum Reprod. 2024 Feb 1;39(2):303-309

Previous relevant publications

Ian D. Hickson

Rahul Bhowmick, Mads Lerdrup, Sampath Amitash Gadi, Giacomo G. Rossetti, Manika I. Singh, Ying Liu, Thanos D. Halazonetis, Ian D. Hickson. RAD51 protects human cells from transcription-replication conflicts. Molecular Cell, 2022, 2022 Aug 18;S1097-2765(22)00706-7.
Meijering, A.E.C., Sarlos, K., Nielsen, C.F., Witt, H., Harju, J., Kerklingh, E., Haasnoot, G.H., Bizard, A.H., Heller, I., Broedersz, C.P., Liu, Y., Peterman, E.J.G., Hickson, I.D.* and Wuite, G.J.L.* (2022) Nonlinear mechanics of human mitotic chromosomes. Nature 605, 545-550. *Co-corresponding authors.
Macheret,M., Bhowmick, R., Sobkowiak, K., Padayachy, L., Mailler, J., Hickson, I.D.* and Halazonetis, T.D.* (2020) High resolution mapping of mitotic DNA synthesis regions and common fragile sites in the human genome through direct sequencing. Cell Research 30, 997-1008, *Co-corresponding authors.
Bizard, A.H., Allemand, J-F., Hassenkam, T., Paramasivam, M., Sarlós, K., Singh, M.I. and Hickson, I.D. (2019) PICH and TOP3A cooperate to induce positive DNA supercoiling. Nature Struct. Molec. Biol., 26, 267-274.
Sarlós, K., Biebricher, A.S., Bizard, A.H., Bakx, J.A.M., Ferrete-Bonastre, A.G., Modesti, M., Paramasivam, M., Yao, Q., Peterman, E.J.G., Wuite, G.J.L. and Hickson, I.D. (2018) Reconstitution of anaphase DNA bridge recognition and disjunction in vitro. Nature Structural and Molecular Biology 25, 868-876.
Bhowmick R, Minocherhomji S, Hickson ID. RAD52 Facilitates Mitotic DNA Synthesis Following Replication Stress. Mol Cell. 2016 Dec 15;64(6):1117-1126.
Minocherhomji, S., Ying, S., Bjerregaard, V.A., Bursomanno, S., Aleliunaite, A., Wu, W., Mankouri, H.W., Shen, H., Liu, Y. and Hickson, I.D. (2015) Replication stress activates DNA repair synthesis in mitosis. Nature 528, 286-290.

Eva Hoffmann

Copsey A, Tang S, Jordan PW, Blitzblau HG, Newcombe S, Chan AC, Newnham L, Li Z, Gray S, Herbert AD, Arumugam P, Hochwagen A, Hunter N, Hoffmann E. Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions.PLoS Genet. 2013;9(12):e1004071.
Sarkar S, Shenoy RT, Dalgaard JZ, Newnham L, Hoffmann E, Millar JB, Arumugam P. Monopolin subunit Csm1 associates with MIND complex to establish monopolar attachment of sister kinetochores at meiosis I. PLoS Genet. 2013;9(7):e1003610
Newnham L, Jordan P, Rockmill B, Roeder GS, Hoffmann E. The synaptonemal complex protein, Zip1, promotes the segregation of nonexchange chromosomes at meiosis I. Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):781-5
Falk JE, Chan AC, Hoffmann E, Hochwagen A A Mec1- and PP4-dependent checkpoint couples centromere pairing to meiotic recombination. Dev Cell. 2010 Oct 19;19(4):599-611
Jordan P, Copsey A, Newnham L, Kolar E, Lichten M, Hoffmann E. Ipl1/Aurora B kinase coordinates synaptonemal complex disassembly with cell cycle progression and crossover formation in budding yeast meiosis. Genes Dev. 2009 Sep 15;23(18):2237-51

Mads Lerdrup

Bhowmick R, Mehta KPM, Lerdrup M, Cortez D. Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability. Mol Cell. 2023, 83(13):2357-2366.e8. doi: 10.1016/j.molcel.2023.05.015.
Li Y, Wang Y, Vera-Rodriguez M, Lindeman LC, Skuggen LE, Rasmussen EMK, Jermstad I, Khan S, Fosslie M, Skuland T, Indahl M, Khodeer S, Klemsdal EK, Jin KX, Dalen KT, Fedorcsak P, Greggains GD, Lerdrup M, Klungland A, Au KF, Dahl JA. Single-cell m6A mapping in vivo using picoMeRIP-seq. Nature Biotech. 2023, doi: 10.1038/s41587-023-01831-7.
Hansen SL, Larsen HL, Pikkupeura LM, Maciag G, Guiu J, Müller I, Clement DL, Mueller C, Johansen JV, Helin K, Lerdrup M, Jensen KB, An organoid-based CRISPR/Cas9 screen for regulators of intestinal epithelial maturation. Science Adv. 2023, 9(28):eadg4055. doi: 10.1126/sciadv.adg4055.
Maynard S, Hall S, Galanos P, Rizza S, Yamamoto T, Gram HH, Munk SHN, Shoaib M, Sørensen CS, Bohr VA, Lerdrup M*, Maya-Mendoza A*, Bartek J. Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGC1a and the NAMPT-NAD+ pathway. Nucleic Acids Res. 2022, 50 (17), 9948-9965. *) Senior authors
Bhowmick R*, Lerdrup M*, Gadi SA, Rossetti GG, Singh MI, Liu Y, Halazonetis TD, Hickson ID. RAD51 protects human cells from transcription-replication conflicts. Mol Cell. 2022 Aug 18:S1097-2765(22)00706-7. doi: 10.1016/j.molcel.2022.07.010. *) Shared first-authors
Zonderland G, Vanzo R, Gadi SA, Coscia F, Mund A, Lerdrup M, Benada J, Boos D, Toledo LIT. The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition. Mol Cell. 2022. 82(18):3350-3365.e7. doi: 10.1016/j.molcel.2022.08.006.
Sankar A, Mohammad F, Sundaramurthy AK, Wang H, Lerdrup M, Tatar T, Helin K. Histone editing elucidates the functional roles of H3K27 methylation and acetylation in mammals. Nat Genet. 2022 Jun;54(6):754-760. doi: 10.1038/s41588-022-01091-2.
Shoaib S, Chen Q, Shi X , Nair N, Prasanna C, Yang R, Walter D, Frederiksen KS, Einarsson H, Svensson JP, Liu CF, Ekwall K, Lerdrup M*, Nordenskiöld L*, Sørensen CS*. Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes. Nature Commun. 2021 Aug 20;12(1):4800. doi: 10.1038/s41467-021-25051-2. *) Corresponding authors
Sankar A*, Lerdrup M*, Manaf A*, Johansen JV, Gonzalez JM, Borup R, Blanshard R, Klungland A, Hansen K, Andersen CY, Dahl JA, Helin K, Hoffmann ER. KDM4A regulates the maternal-to-zygotic transition by protecting broad H3K4me3 domains from H3K9me3 invasion in oocytes. Nature Cell Biol. 2020 Apr;22(4):380-388. doi: 10.1038/s41556-020-0494-z. *) Shared first-authors
Dahl JA, Jung I, Aanes H, Greggains GD, Manaf A, Lerdrup M, Li G, Kuan S, Li B, Lee AY, Preissl S, Jermstad I, Haugen MH, Suganthan R, Bjørås M, Hansen K, Dalen KT, Fedorcsak P, Ren B, Klungland A. Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition. Nature. 2016 Sep 22;537(7621):548-552. doi: 10.1038/nature19360.
Lerdrup M*, Johansen JV, Agrawal-Singh S, Hansen K*. An interactive environment for agile analysis and visualization of ChIP-sequencing data. Nature Struct Mol Biol. 2016 Apr;23(4):349-57. doi: 10.1038/nsmb.3180. *) Corresponding authors

Michael Lisby

Brannvoll A, Xue X, Kwon Y, Kompocholi S, Simonsen AKW, Viswalingam KS, Gonzalez L, Hickson ID, Oestergaard VH, Mankouri HW, Sung P, Lisby M. The ZGRF1 Helicase Promotes Recombinational Repair of Replication-Blocking DNA Damage in Human Cells. Cell Rep. 2020 Jul 7;32(1):107849. doi: 10.1016/j.celrep.2020.107849. PMID: 32640219; PMCID: PMC7473174
Bagge J, Oestergaard VH, Lisby M. Functions of TopBP1 in preserving genome integrity during mitosis. Semin Cell Dev Biol. 2021 May;113:57-64. doi: 10.1016/j.semcdb.2020.08.009. Epub 2020 Sep 8. PMID: 32912640.
Germann SM, Schramke V, Pedersen RT, Gallina I, Eckert-Boulet N, Oestergaard VH, Lisby M.TopBP1/Dpb11 binds DNA anaphase bridges to prevent genome instability. J Cell Biol. 2014 Jan 6;204(1):45-59
Nielsen I, Bentsen IB, Lisby M, Hansen S, Mundbjerg K, Andersen AH, Bjergbaek L. A Flp-nick system to study repair of a single protein-bound nick in vivo. Nat Methods. 2009 Oct;6(10):753-7
Khadaroo B, Teixeira MT, Luciano P, Eckert-Boulet N, Germann SM, Simon MN, Gallina I, Abdallah P, Gilson E, Géli V, Lisby M. The DNA damage response at eroded telomeres and tethering to the nuclear pore complex. Nat Cell Biol. 2009 Aug;11(8):980-7
Pedersen, R. T., T. Kruse, J. Nilsson, V. H. Oestergaard and M. Lisby, 2015. TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells. J Cell Biol 210: 565-582.
Lisby M, Barlow JH, Burgess RC, Rothstein R. Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins. Cell. 2004 Sep 17;118(6):699-713.

Ying Liu

Wu W, Barwacz SA, Bhowmick R, Lundgaard K, Gonçalves Dinis MM, Clausen M, Kanemaki MT, Liu, Y. Mitotic DNA synthesis in response to replication stress requires the sequential action of DNA polymerases zeta and delta in human cells. Nature Communications 2023 Feb 9;14(1):706. doi: 10.1038/s41467-023-35992-5.
Meijering AEC, Sarlos K, Nielsen CF, Witt H, Harju J, Kerklingh E, Haasnoot GH, Bizard AH, Heller I, Broedersz CP*, Liu Y, Peterman E J G, Hickson I D*, Wuite G J L*. Nonlinear mechanics of human mitotic chromosomes. Nature 2022 605, 545-550. (*:corresponding authors)
Wu W^$, Bhowmick R^$, Vogel I, Ozer O, Ghisays F, Thakur RS, Sanchez de Leon E, Richter PH, Ren L, Petrini JH, Hickson ID*, and Liu Y*, RTEL1 suppresses G-quadruplex-associated R-loops at difficult-to-replicate loci in the human genome. Nature Structural & Molecular Biology 2020. 27(5): p. 424-437. (^$: Joint authorship; *:corresponding authors)
Garribba L, Bjerregaard VA, Goncalves Dinis MM, Ozer O, Wu W, Sakellariou D, Pena-Diaz J, Hickson ID, and Liu Y, Folate stress induces SLX1- and RAD51-dependent mitotic DNA synthesis at the fragile X locus in human cells. Proc Natl Acad Sci U S A, 2020. 117(28): p. 16527-16536.
Bjerregaard VA, Garribba L, McMurray CT, Hickson ID, Liu Y. Folate deficiency drives mitotic missegregation of the human FRAXA locus. Proc Natl Acad Sci USA. 2018 Dec 115 (51) 13003-13008
Garribba L*, Wu W*, Özer Ö, Bhowmick R, Hickson ID, Liu, Y. Inducing and Detecting Mitotic DNA Synthesis at Difficult-to-Replicate Loci. Methods Enzymol. 2018;601:45-58. (*: Joint authorship)
Bjerregaard VA, Özer Ö, Hickson ID, Liu Y. The Detection and Analysis of Chromosome Fragile Sites. Methods in Molecular Biology. 2018;1672:471-482
Ren L, Chen L, Wu W, Garribba L, Tian H, Liu Z, Vogel I, Li C, Hickson ID, Liu Y. Potential biomarkers of DNA replication stress in cancer. Oncotarget. 2017 Jun 6;8(23):36996-37008
Minocherhomji S*, Ying SM*, Bjerregaard VA, Bursomanno S, Aleliunaite A, Wu W, Mankouri HW, Shen HH^§, Liu Y^§, Hickson ID^§. Replication Stress Activates DNA Repair Synthesis in Mitosis. Nature, 2015 Dec.; 528(7581):286-90. (*: joint authorship; ^§: corresponding authors)
Bursomanno S, Beli P, Khan AM, Minocherhomji S, Wagner SA, Bekker-Jensen S, Mailand N, Choudhary C, Hickson ID, Liu Y. Proteome-wide analysis of SUMO2 targets in response to pathological DNA replication stress in human cells. DNA Repair, 2015 (25): 84-96
Bursomanno S, McGouran JF, Kessler BM, Hickson ID, and Liu Y. Regulation of SUMO2 target proteins by the proteasome in human cells exposed to replication stress Journal of Proteome Research, 2015 Apr 3;14(4):1687-99
Liu Y, Nielsen CF, Yao Q, Hickson ID. The origins and processing of ultra-fine anaphase DNA bridges. Current Opinion in Genetics & Development 2014 Apr 30;26:1-5
Ying S, Minocherhomji S, Chan KL, Palmai-Pallag T, Chu WK, Wass T, Mankouri HW, Liu Y, Hickson ID. MUS81 promotes common fragile site expression. Nature Cell Biology, 2013 Aug;15(8):1001-7
Wilding JL, McGowan S, Liu Y, Bodmer WF, Replication error deficient and proficient colorectal cancer gene expression differences caused by 3'UTR polyT sequence deletions. Proc Natl Acad Sci U S A. 2010 Dec 7;107(49):21058-63
Liu Y, and Bodmer WF. Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines. Proc Natl Acad Sci USA, 2006, 103(4):976-81

Andrés Lopez-Contreras

Atashpaz S, Samadi Shams S, Gonzalez JM, Sebestyén E, Arghavanifard N, Gnocchi A, Albers E, Minardi S, Faga G, Soffientini P, Allievi E, Cancila V, Bachi A, Fernández-Capetillo Ó, Tripodo C, Ferrari F, López-Contreras AJ, Costanzo V. ATR expands embryonic stem cell fate potential in response to replication stress. Elife. 2020 Mar 12;9. pii: e54756.
Pladevall-Morera D, Munk S, Ingham A, Garribba L, Albers E, Liu Y, Olsen JV, Lopez-Contreras AJ. Proteomic characterization of chromosomal common fragile site (CFS)-associated proteins uncovers ATRX as a regulator of CFS stability.Nucleic Acids Res. 2019 Sep 5;47(15):8004–8018
Albers E, Sbroggiò M, Pladevall-Morera D, Bizard AH, Avram A, Gonzalez P, Martin-Gonzalez J, Hickson ID, Lopez-Contreras AJ. Loss of PICH Results in Chromosomal Instability, p53 Activation, and Embryonic Lethality.
Cell Rep (2018)
Lopez-Contreras AJ, Specks J, Barlow JH, Ambrogio C, Desler C, Vikingsson S, Rodrigo-Perez S, Green H, Rasmussen LJ, Murga M, Nussenzweig A, Fernandez-Capetillo O Increased Rrm2 gene dosage reduces fragile site breakage and prolongs survival of ATR mutant mice. Genes Dev. 2015 Apr 1;29(7):690-5.
Lopez-Contreras AJ, Ruppen I, Nieto-Soler M, Murga M, Rodriguez-Acebes S, Remeseiro S, Rodrigo-Perez S, Rojas AM, Mendez J, Muñoz J, Fernandez-Capetillo O. A proteomic characterization of factors enriched at nascent DNA molecules. Cell Rep. 2013 Apr 25;3(4):1105-16
Marqués-Torrejón MÁ, Porlan E, Banito A, Gómez-Ibarlucea E, Lopez-Contreras AJ, Fernández-Capetillo O, Vidal A, Gil J, Torres J, Fariñas I. Cyclin-dependent kinase inhibitor p21 controls adult neural stem cell expansion by regulating Sox2 gene expression. Cell Stem Cell. 2013 Jan 3;12(1):88-100
López-Contreras AJ, Gutierrez-Martinez P, Specks J, Rodrigo-Perez S, Fernandez-Capetillo O. An extra allele of Chk1 limits oncogene-induced replicative stress and promotes transformation. J Exp Med. 2012 Mar 12;209(3):455-61
Murga M, Campaner S, Lopez-Contreras AJ, Toledo LI, Soria R, Montaña MF, D'Artista L, Schleker T, Guerra C, Garcia E, Barbacid M, Hidalgo M, Amati B, Fernandez-Capetillo O. Exploiting oncogene-induced replicative stress for the selective killing of Myc-driven tumors. Nat Struct Mol Biol. 2011 Nov 27;18(12):1331-5
López-Contreras AJ, Fernandez-Capetillo O. The ATR barrier to replication-born DNA damage. DNA Repair (Amst). 2010 Dec 10;9(12):1249-55

Niels Mailand

Haahr P, Borgermann N, Guo X, Typas D, Achuthankutty D, Hoffmann S, Shearer R, Sixma TK, Mailand N. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability. Mol Cell. 2018 Apr 5;70(1):165-174.e6.
Raschle M, Smeenk G, Hansen RK, Temu T, Oka Y, Hein MY, Nagaraj N, Long DT, Walter JC, Hofmann K, Storchova Z, Bekker-Jensen S, Mailand N, Mann M. (2015). Proteomics reveals dynamic assembly of repair complexes during bypass of DNA crosslinks. Science, 384, 1253671.
Gibbs-Seymour I, Oka Y, Rajendra E, Weinert BT, Passmore LA, Patel KJ, Olsen JV, Choudhary C, Bekker-Jensen S, Mailand N. (2014). Ubiquitin-SUMO circuitry controls activated Fanconi Anemia ID complex dosage in response to DNA damage. Mol Cell, 57, 150-164.
Mosbech A, Gibbs-Seymour I, Kagias K, Thorslund T, Beli P, Povlsen LK, Nielsen SV, Smedegaard S, Sedgwick G, Lukas C, Petersen RH, Lukas J, Choudhary C, Pocock R, Bekker-Jensen S, Mailand N. (2012). DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks. Nature Struct. Mol. Biol., 19, 1084-1092.
Povlsen, LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C. (2012). Systems-wide analysis of ubiquitylation dynamics reveals a key role of PAF15 ubiquitylation in DNA damage bypass. Nature Cell Biol., 14, 1089-1098.
Bekker-Jensen S, Danielsen JR, Fugger K, Gromova I, Nerstedt A, Bartek J, Lukas J, Mailand N. (2010). HERC2 coordinates ubiquitin-dependent assembly of DNA repair factors on damaged chromosomes. Nature Cell Biol., 12, 80-86

Tom Miller

Lim CT^†, Miller TCR^†^*, Tan KW, Talele S, Early A, East P, Sánchez H, Dekker NH, Costa A, Diffley JFX^†*, Cell Cycle Regulation has Shaped Budding Yeast Replication Origin Structure and Function, bioRxiv 2024.01.10.575016 (preprint). ^†Equal contribution. ^*Corresponding authors.
Sellés-Baiget S, Ambjørn SA, Carli A, Hendriks IA, Gallina I, Davey NE, Benedict B, Meeusen B, Hertz EPT, Slappendel L, Semlow D, Gadi SA, Sturla S, Nielsen ML, Nilsson J, Miller TCR, Duxin JP, Catalytic and non-catalytic functions of DNA polymerase kappa in translesion DNA synthesis, bioRxiv 2023.11.06.565773 (preprint)

Polasek-Sedlackova H, Miller TCR, Krejci J, Rask MB, Lukas J Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes. Nature Communications. 2022 Oct 14;13(1):6090.
Pühringer T, Greiwe JF, Miller TCR*, Costa A*. ReconSil: An electron microscopy toolbox to study helicase function at an origin of replication. Methods in Enzymology, Academic Press, 2022, ISSN 0076-6879, *Corresponding authors
Greiwe JF, Zanetti, G, Miller TCR*, Costa A*.“In silico reconstitution of DNA replication. Lessons from single-molecule imaging and cryo-tomography applied to single-particle cryo-EM. Current Opinion in Structural Biology, 2022, Jan 10; 72, 279-286. *Corresponding authors.
Greiwe JF, Miller TCR, Locke J, Martino F, Howell S, Schreiber A, Nans A, Diffley JFX, Costa A. Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase. Nature Structural and Molecular Biology, 2021 Dec 28.
Miller TCR, Locke J, Greiwe JF, Diffley JFX, Costa A. Mechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM. Nature, 2019 Nov 20; 575, 704-710.
Miller TCR, Costa A. The architecture and function of the chromatin replication machinery. Current Opinion in Structural Biology, 2017 Dec 1; 47, 9-16.

Previous publications from CCS

Publications 2023

Ummethum H, Li J, Lisby M, Oestergaard VH, Emerging roles of the CIP2A-TopBP1 complex in genome integrity. NAR Cancer. 2023 Oct 11;5(4):zcad052
Bhowmick R, Hickson ID, Liu Y, Completing genome replication outside of S phase. Mol Cell. 2023 Sep 8:S1097-2765(23)00659-7.
Hertz EPT, Vega IA, Kruse T, Wang Y, Hendriks IA, Bizard AH, Eugui-Anta A, Hay RT, Nielsen ML, Nilsson J, Hickson ID, Mailand N. The SUMO-NIP45 pathway processes toxic DNA catenanes to prevent mitotic failure. Nat Struct Mol Biol. 2023 Sep;30(9):1303-1313.
Knoblochova L, Duricek T, Vaskovicova M, Zorzompokou C, Rayova D, Ferencova I, Baran V, Schultz RM, Hoffmann ER, Drutovic D. CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos.EMBO Rep. 2023 Sep 11:e56530.
Hoffmann E, Andersen PT, Mogensen CB, Prinds C, Primdahl J. Relatives' negotiations with healthcare professionals during older people's admission in an emergency department: An ethnographic study. Nurs Open. 2023 Sep;10(9):6381-6389.
Asserhøj LL, Mizrak I, Heldarskard GF, Clausen TD, Hoffmann ER, Greisen G, Main KM, Madsen PL, Jensen RB, Pinborg A. Childhood BMI after ART with frozen embryo transfer. Hum Reprod. 2023 Aug 1;38(8):1578-1589.
Shekari S, Stankovic S, Gardner EJ, Hawkes G, Kentistou KA, Beaumont RN, Mörseburg A, Wood AR, Prague JK, Mishra GD, Day FR, Baptista J, Wright CF, Weedon MN, Hoffmann ER, Ruth KS, Ong KK, Perry JRB, Murray A. Penetrance of pathogenic genetic variants associated with premature ovarian insufficiency. Nat Med. 2023 Jul;29(7):1692-1699.
Tsui V, Lyu R, Novakovic S, Stringer JM, Dunleavy JEM, Granger E, Semple T, Leichter A, Martelotto LG, Merriner DJ, Liu R, McNeill L, Zerafa N, Hoffmann ER, O'Bryan MK, Hutt K, Deans AJ, Heierhorst J, McCarthy DJ, Crismani W. Fancm has dual roles in the limiting of meiotic crossovers and germ cell maintenance in mammals. Cell Genom. 2023 Jun 29;3(8):100349.
Mathieson I, Day FR, Barban N, Tropf FC, Brazel DM; eQTLGen Consortium; BIOS Consortium; Vaez A, van Zuydam N, Bitarello BD, Gardner EJ, Akimova ET, Azad A, Bergmann S, Bielak LF, Boomsma DI, Bosak K, Brumat M, Buring JE, Cesarini D, Chasman DI, Chavarro JE, Cocca M, Concas MP, Davey Smith G, Davies G, Deary IJ, Esko T, Faul JD; FinnGen Study; Franco O, Ganna A, Gaskins AJ, Gelemanovic A, de Geus EJC, Gieger C, Girotto G, Gopinath B, Grabe HJ, Gunderson EP, Hayward C, He C, van Heemst D, Hill WD, Hoffmann ER, Homuth G, Hottenga JJ, Huang H, Hyppӧnen E, Ikram MA, Jansen R, Johannesson M, Kamali Z, Kardia SLR, Kavousi M, Kifley A, Kiiskinen T, Kraft P, Kühnel B, Langenberg C, Liew G; Lifelines Cohort Study; Lind PA, Luan J, Mägi R, Magnusson PKE, Mahajan A, Martin NG, Mbarek H, McCarthy MI, McMahon G, Medland SE, Meitinger T, Metspalu A, Mihailov E, Milani L, Missmer SA, Mitchell P, Møllegaard S, Mook-Kanamori DO, Morgan A, van der Most PJ, de Mutsert R, Nauck M, Nolte IM, Noordam R, Penninx BWJH, Peters A, Peyser PA, Polašek O, Power C, Pribisalic A, Redmond P, Rich-Edwards JW, Ridker PM, Rietveld CA, Ring SM, Rose LM, Rueedi R, Shukla V, Smith JA, Stankovic S, Stefánsson K,…See abstract for full author list ➔. Genome-wide analysis identifies genetic effects on reproductive success and ongoing natural selection at the FADS locus. Nat Hum Behav. 2023 May;7(5):790-801
Schlaikjær Hartwig T, Ambye L, Gruhn JR, Petersen JF, Wrønding T, Amato L, Chi-Ho Chan A, Ji B, Bro-Jørgensen MH, Werge L, Petersen MMBS, Brinkmann C, Petersen JB, Dunø M, Bache I, Herrgård MJ, Jørgensen FS, Hoffmann ER, Nielsen HS; COPL consortium. Cell-free fetal DNA for genetic evaluation in Copenhagen Pregnancy Loss Study (COPL): a prospective cohort study. Lancet. 2023 Mar 4;401(10378):762-771.
Sørensen SG, Shrikhande A, Poulsgaard GA, Christensen MH, Bertl J, Laursen BE, Hoffmann ER, Pedersen JS. Pan-cancer association of DNA repair deficiencies with whole-genome mutational patterns. Elife. 2023 Mar 8;12:e81224.
Wu W, Barwacz SA, Bhowmick R, Lundgaard K, Gonçalves Dinis MM, Clausen M, Kanemaki MT, Liu Y. Mitotic DNA synthesis in response to replication stress requires the sequential action of DNA polymerases zeta and delta in human cells. Nat Commun. 2023 Feb 9;14(1):706.

Publications 2022

Geng, X., Zhang, C., Wang, J., Ji, F., Li, M., Feng, H., Xing, M., Zhang, L., Li, W., Chen, Z., Hickson, I.D.*, Shen, H.* and Ying, S.* (2022) PICH supports embryonic hematopoiesis by suppressing a cGas-Sting-mediated interferon response. Advanced Science 9, 2103837. *Co-corresponding authors.
Spakman, D., Clement, T.V.M., Biebricher, A.S., King, G.A., Singh, M.I., Hickson, I.D., Peterman, E.J.G. and Wuite, G.J.L. (2022) PICH acts as a force-dependent nucleosome remodeler. Nature Communications 13, 7277.
Hoffmann E, Andersen PT, Mogensen CB, Prinds C, Primdahl J. Relatives' negotiation power in relation to older people's acute hospital admission: A qualitative interview study. Scand J Caring Sci. 2022 Dec;36(4):1016-1026.
Gruhn JR, Hoffmann ER. Errors of the Egg: The Establishment and Progression of Human Aneuploidy Research in the Maternal Germline. Annu Rev Genet. 2022 Nov 30;56:369-390. Review.
Hoffmann E, Wilburn-Wren K, Dillon SJ, Barahona A, McIntire DD, Nelson DB. Impact of implementation of the Maternal-Fetal Triage Index on patients presenting with severe hypertension. Am J Obstet Gynecol. 2022 Sep;227(3):521.e1-521.e8.
Capalbo A, Buonaiuto S, Figliuzzi M, Damaggio G, Girardi L, Caroselli S, Poli M, Patassini C, Cetinkaya M, Yuksel B, Azad A, Grøndahl ML, Hoffmann ER, Simón C, Colonna V, Kahraman S. Maternal exome analysis for the diagnosis of oocyte maturation defects and early embryonic developmental arrest. Reprod Biomed Online. 2022 Sep;45(3):508-518.
Wang D, Hildorf S, Ntemou E, Dong L, Pors SE, Mamsen LS, Fedder J, Hoffmann ER, Clasen-Linde E, Cortes D, Thorup J, Andersen CY. Characterization and Survival of Human Infant Testicular Cells After Direct Xenotransplantation. Front Endocrinol (Lausanne). 2022 Mar 10;13:853482.
Jensen, C. F. S., Mamsen, L. S., Wang, D., Fode, M., Giwercman, A., Jørgensen, N., Ohl, D., Fedder, J., Hoffmann, E. R., Andersen, C. Y., & Sønksen, J. O. R. (2022). Male fertility restoration - results from the first autologous grafting of human testis tissue. Abstract from Danish Urological Association 2022, Kolding, Denmark.
Jensen CFS, Dong L, Gul M, Fode M, Hildorf S, Thorup J, Hoffmann E, Cortes D, Fedder J, Andersen CY, Sønksen J. Fertility preservation in boys facing gonadotoxic cancer therapy. Nat Rev Urol. 2022 Feb;19(2):71-83. Review
Maynard S, Hall A, Galanos P, Rizza S, Yamamoto T, Gram HH, Munk SHN, Shoaib M, Sørensen CS, Bohr VA, Lerdup M. Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGCa and the NAMPT-NAD+ pathway. Nucleic Acids Research, 2022, Vol 50, 17, 9948-9965
Polasek-Sedlackova H, Miller TCR, Krejci J, Rask MB, Lukas J Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes. Nature Communications. 2022 Oct 14;13(1):6090.
Zonderland G, Vanzo R, Amitash S, Martín-Doncel E, Coscia F, Mund A, Lerdrup M, Benada J, Boos D, Toledo L.
The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition. Molecular Cell. 2022 Sep 15;82(18):3350-3365.e7.
Rahul Bhowmick, Mads Lerdrup, Sampath Amitash Gadi, Giacomo G. Rossetti, Manika I. Singh, Ying Liu, Thanos D. Halazonetis, Ian D. Hickson. RAD51 protects human cells from transcription-replication conflicts. Molecular Cell, 2022, 2022 Aug 18;S1097-2765(22)00706-7.
Meijering, A.E.C., Sarlos, K., Nielsen, C.F., Witt, H., Harju, J., Kerklingh, E., Haasnoot, G.H., Bizard, A.H., Heller, I., Broedersz, C.P., Liu, Y., Peterman, E.J.G., Hickson, I.D.* and Wuite, G.J.L.* (2022) Nonlinear mechanics of human mitotic chromosomes. Nature 605, 545-550. *Co-corresponding authors.
Vajen B, Bhowmick R, Greiwe L, Schäffer V, Eilers M, Reinkens T, Stalke A, Schmidt G, Fiedler J, Thum T, DeLuca DS, Hickson ID, Schlegelberger B, Illig T, Skawran B. MicroRNA-449a Inhibits Triple Negative Breast Cancer by Disturbing DNA Repair and Chromatid Separation.Int J Mol Sci. 2022 May 4;23(9):5131.
Pühringer T, Greiwe JF, Miller TCR*, Costa A*. ReconSil: An electron microscopy toolbox to study helicase function at an origin of replication. Methods in Enzymology, Academic Press, 2022, ISSN 0076-6879, *Corresponding authors
Schubert L, Hendriks IA, Hertz EPT, Wu W, Sellés-Baiget S, Hoffmann S, Viswalingam KS, Gallina I, Pentakota S, Benedict B, Johansen J, Apelt K, Luijsterburg MS, Rasmussen S, Lisby M, Liu Y, Nielsen ML, Mailand N, Duxin JP. SCAI promotes error-free repair of DNA interstrand crosslinks via the Fanconi anemia pathway. .EMBO Rep. 2022 Apr 5;23(4):e53639.
Jensen CFS, Dong L, Gul M, Fode M, Hildorf S, Thorup J, Hoffmann E, Cortes D, Fedder J, Andersen CY, Sønksen J. Fertility preservation in boys facing gonadotoxic cancer therapy. Nat Rev Urol. 2022 Feb;19(2):71-83. Review
Greiwe JF, Zanetti G, Miller TCR, Costa A. In silico reconstitution of DNA replication. Lessons from single-molecule imaging and cryo-tomography applied to single-particle cryo-EM. Curr Opin Struct Biol. 2022 Jan 10;72:279-286. Review
Greiwe JF, Miller TCR, Locke J, Martino F, Howell S, Schreiber A, Nans A, Diffley JFX, Costa A. Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase. Nat Struct Mol Biol. 2022 Jan;29(1):10-20.
Bakx JAM, Biebricher AS, King GA, Christodoulis P, Sarlós K, Bizard AH, Hickson ID, Wuite GJL, Peterman EJG. Duplex DNA and BLM regulate gate opening by the human TopoIIIα-RMI1-RMI2 complex. Nat Commun. 2022 Jan 31;13(1):584.

Publications 2021

Wiegard A, Kuzin V, Cameron DP, Grosser J, Ceribelli M, Mehmood R, Ballarino R, Valant F, Grochowski R, Karabogdan I, Crosetto N, Lindqvist A, Bizard AH, Kouzine F, Natsume T, Baranello L. Topoisomerase 1 activity during mitotic transcription favors the transition from mitosis to G1. Mol Cell. 2021 Dec 16;81(24):5007-5024.e9.
Capalbo A, Poli M, Rienzi L, Girardi L, Patassini C, Fabiani M, Cimadomo D, Benini F, Farcomeni A, Cuzzi J, Rubio C, Albani E, Sacchi L, Vaiarelli A, Figliuzzi M, Findikli N, Coban O, Boynukalin FK, Vogel I, Hoffmann E, Livi C, Levi-Setti PE, Ubaldi FM, Simón C. Mosaic human preimplantation embryos and their developmental potential in a prospective, non-selection clinical trial. Am J Hum Genet. 2021 Dec 2;108(12):2238-2247.
Liu JCY, Kühbacher U, Larsen NB, Borgermann N, Garvanska DH, Hendriks IA, Ackermann L, Haahr P, Gallina I, Guérillon C, Branigan E, Hay RT, Azuma Y, Nielsen ML, Duxin JP, Mailand N. Mechanism and function of DNA replication-independent DNA-protein crosslink repair via the SUMO-RNF4 pathway. EMBO J. 2021 Sep 15;40(18):e107413
Shoaib M, Chen Q, Shi X, Nair N, Prasanna C, Yang R, Walter D, Frederiksen KS, Einarsson H, Svensson JP, Liu CF, Ekwall K, Lerdrup M, Nordenskiöld L, Sørensen CS Histone H4 lysine 20 mono-methylation directly facilitates chromatin openness and promotes transcription of housekeeping genes. Nat Commun. 2021 Aug 20;12(1):4800
Halliwell JA, Hoffmann ER, First come, first served: Mammalian recombination is timed to replication, Cell. 2021 Aug 5;184(16):4112-4114.
Garribba, L., Vogel, I., Lerdrup, M., Goncalves Dinis, M.M., Ren, L., and Liu, Y. (2021). Folate Deficiency Triggers the Abnormal Segregation of a Region With Large Cluster of CG-Rich Trinucleotide Repeats on Human Chromosome 2. Front Genet. 2021 Jul 1;12:695124
Roy U, Kwon Y, Marie L, Symington L, Sung P, Lisby M, Greene EC. The Rad51 paralog complex Rad55-Rad57 acts as a molecular chaperone during homologous recombination. Mol Cell. 2021 Mar 4;81(5):1043-1057.e8.
Moreno Pescador GS, Aswad DS, Arastoo MR, Danielsen HMD, Florentsen CD, Bahadori A, Nylandsted J, Oddershede LB, Bendix PM, Investigating Plasma-Membrane Repair Employing Thermoplasmonics. , Feb 2021,Biophysical Journal. 120, 3, Supp. 1, p. 45a-45a 1 p., 221-Pos
Capalbo A, Poli M, Riera-Escamilla A, Shukla V, Kudo Høffding M, Krausz C, Hoffmann ER, Simon C. Preconception genome medicine: current state and future perspectives to improve infertility diagnosis and reproductive and health outcomes based on individual genomic data. Hum Reprod Update. 2021 Feb 19;27(2):254-279.
Vogel, I., Cai, L., Jerman-Plesec, L., and Hoffmann, E. R.(2021). “SureTypeSCR: R package for rapid quality control and genotyping of SNP arrays from single cells”. F1000Research, in press.
Ruth*, Day*, Hussain*, Martinez-Marchal*,….Hoffmann, E.R*., Murray, A*., Roig, I*., and Perry, J.R.P.* (in press). “Genetic insights into the biological mechanisms governing human ovarian ageing”. Nature, in press. *equal contributions.
Hassold, T., Maylor-Hagen, H., Wood, A., Gruhn, J., Hoffmann, E., Broman, K. W., and Hunt, P. (2021). “Failure to recombination is a common feature of human oogenesis”. American Journal of Human Genetics, 108: 16-24.
Egerup, P., Olsen, L. F., Christiansen, A.M.H., Westergaard, D., Severinsen, E. R., Hviid K. V. R., Kolte, A. M., Boje, A. D., Bertelsen, A.L.M.F., Prætorius, L., Zedeler, A., Nielsen, J. R., Bang, D., Berntsen, S., Ethelberg-Findsen, J., Storm, D. M. Bello-Rodrigues, J., Ingham, A., Olle-Lopez, J., Hoffmann, E. R., Wilken-Jensen, C., Krebs, L., Jørgensen, F. S., Westh, H., Jørgsensen, H. L., Freisesleben, N.L.C., and Nielsen, H. S. (2021). Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Antibodies at Delivery in Women, Partners, and Newborns. Obstetrics and Gynaecology 137: 49-55.
La Cour Freiesleben, Egerup, N., Christianssen, A-M., H., Westergaard, D., …..Hoffmann, E.R., Wilken-Jensen, C., Krebs, L., Jørgensen, F.S-, Westh, H., Jørgensen, H.L., la Cour Freiesleben, N., and Nielsen, H. S. (2021). “SARS-CoV-2 in first trimester pregnancy: a cohort study”. Human Reproduction 36: 40-47.
Olsen, K. W., Castillo-Fernandez, J., Chan, Chi-ho, Freiesleben, N. l. C., Zedeler, A., Bungum, M., Cardona, A., Perry, J. R. B., Skouby, S. O., Hoffmann, E. R., Kelsey ,G., and Grøndahl, M. L. (2021). “Identification of a unique epigenetic profile in women with diminished ovarian reserved”. Fertility and Sterility 115: 732-741.
Levy, B., Hoffmann, E.R., McCoy, R. C., and Grati, F. R. (2021). “Chromosomal mosaicism: origins and clinical implications in preimplantation and prenatal diagnosis”. Prenatal Diagnosis 41: 631-641.
Lena Wartosch, Karen Schindler, Melina Schuh, Jennifer R. Gruhn, Eva R. Hoffmann*, Rajiv C. McCoy, and Jinchuan Xing (2021). “Origins and mechanisms leading to aneuploidy in human eggs”. Prenatal Diagnosis 41: 620-630.
*corresponding author, author list generated in random order.

Shukla, V. S., Høffding, Hoffmann, E. R. (2021). ”Genome diversity and instability in human germ cells and preimplantation embryos”. Seminars in Cell and Developmental Biology 113: 132-147.

Publications 2020

Bagge, J., and Lisby, M. (2020) Functions of TopBP1 in preserving genome integrity during mitosis, Seminars in Cell and Developmental Biology, S1084-9521(19)30094-1.
Brannvoll, A., Xue, X., Kwon, Y., Kompocholi, S., Simonsen, A.K.W., Viswalingam, K.S., Gonzalez, L., Hickson, I.D., Oestergaard, V.H., Mankouri, H.W., Sung, P., and Lisby, M. (2020) The ZGRF1 helicase promotes recombinational repair of replication-blocking DNA damage in human cells, Cell Reports, 32(1):107849.
Lisby, M. and Xue, X. (2020) Protocol for Purification of Human ZGRF1 and its Regulatory Function on RAD51-mediated D-loop Formation, STAR protocols, 1(2):100099.
Hassold T, Maylor-Hagen H, Wood A, Gruhn J, Hoffmann E, Broman KW, Hunt P, Failure to recombine is a common feature of human oogenesis. Am J Hum Genet. 2020 Nov 24:S0002-9297(20)30407-9.
Hoffmann ER, Roig I. Cas9 in Human Embryos: On Target but No Repair. Cell. 2020 Dec 10;183(6):1464-1466.
la Cour Freiesleben N, Egerup P, Vauvert Römmelmayer Hviid K, Rosenbek Severinsen E, Kolte AM, Westergaard D, Fich Olsen L, Prætorius L, Zedeler A, Hellerung Christiansen AM, Reinhardt Nielsen J, Bang D, Berntsen S, Ollé-López J, Ingham A, Bello-Rodríguez J, Marie Storm D, Ethelberg-Findsen J, Hoffmann ER, Wilken-Jensen C, Stener Jørgensen F, Westh H, Løvendahl Jørgensen H, Nielsen HS. SARS-CoV-2 in first trimester pregnancy: a cohort study. Hum Reprod. 2020 Nov 4:deaa311. Online ahead of print
Hildorf S, Cortes D, Gül M, Dong L, Kristensen SG, Jensen CFS, Clasen-Linde E, Fedder J, Andersen CY, Hoffmann ER, Sønksen J, Fossum M, Thorup J, Parental Acceptance Rate of Testicular Tissue Cryopreservation in Danish Boys with Cryptorchidism Sexual Development. 20 okt. 2020, I 13, 5-6, s. 246-257.
Egerup P, Fich Olsen L, Christiansen AH, Westergaard D, Severinsen ER, Hviid KVR, Kolte AM, Boje AD, Bertelsen MMF, Prætorius L, Zedeler A, Nielsen JR, Bang D, Berntsen S, Ethelberg-Findsen J, Storm DM, Bello-Rodríguez J, Ingham A, Ollé-López J, Hoffmann ER, Wilken-Jensen C, Krebs L, Jørgensen FS, Westh H, Jørgensen HL, la Cour Freiesleben N, Nielsen HS Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Antibodies at Delivery in Women, Partners, and Newborns Obstet Gynecol. 2020 Oct 27. Online ahead of print.
Hoffmann S, Pentakota S, Mund A, Haahr P, Coscia F, Gallo M, Mann M, Taylor NM, Mailand N. FAM111 protease activity undermines cellular fitness and is amplified by gain-of-function mutations in human disease. EMBO Rep. 2020 Aug 9:e50662.
Cannavo E, Sanchez A, Anand R, Ranjha L, Hugener J, Adam C, Acharya A, Weyland N, Aran-Guiu X, Charbonnier JB, Hoffmann ER, Borde V, Matos J, Cejka P. Regulation of the MLH1-MLH3 endonuclease in meiosis. Nature. 2020 Aug 19.
Brannvoll, A., Xue, X., Kwon, Y., Kompocholi, S., Simonsen, A.K.W., Viswalingam, K.S., Gonzalez, L., Hickson, I.D., Oestergaard, V.H., Mankouri, H.W., Sung, P., and Lisby, M. (2020) The ZGRF1 helicase promotes recombinational repair of replication-blocking DNA damage in human cells, Cell Reports, 2020 Jul 7;32(1):107849
Garribba L, Bjerregaard VA, Gonçalves Dinis MM, Özer Ö, Wu W, Sakellariou D, Pena-Diaz J, Hickson ID, Liu Y.
Folate stress induces SLX1- and RAD51-dependent mitotic DNA synthesis at the fragile X locus in human cells.
Proc Natl Acad Sci U S A. 2020 Jun 29. Online ahead of print.
Olsen KW, Castillo-Fernandez J, Zedeler A, Freiesleben NC, Bungum M, Chan AC, Cardona A, Perry JRB, Skouby SO, Borup R, Hoffmann ER, Kelsey G, Grøndahl ML. A distinctive epigenetic ageing profile in human granulosa cells. Hum Reprod. 2020 Jun 1;35(6):1332-1345
Macheret M, Bhowmick R, Sobkowiak K, Padayachy L, Mailler J, Hickson ID, Halazonetis TD. High-resolution mapping of mitotic DNA synthesis regions and common fragile sites in the human genome through direct sequencing. Cell Res. 2020 Jun 19. Online ahead of print.
Nielsen CF, Zhang T, Barisic M, Kalitsis P, Hudson DF Topoisomerase IIα is essential for maintenance of mitotic chromosome structure. Proc Natl Acad Sci U S A. 2020 May 15.
Wu W, Bhowmick R, Vogel I, Özer Ö, Ghisays F, Thakur RS, Sanchez de Leon E, Richter PH, Ren L, Petrini JH, Hickson ID, Liu Y. RTEL1 suppresses G-quadruplex-associated R-loops at difficult-to-replicate loci in the human genome. Nature Structural & Molecular Biology. 2020 May;27(5):424-437.
Wu W, Hickson ID, Liu Y. The prevention and resolution of DNA replication–transcription conflicts in eukaryotic cells. Genome Instability & Disease (2020).
Gul M, Hildorf S, Dong L, Thorup J, Hoffmann ER, Jensen CFS, Sønksen J, Cortes D, Fedder J, Andersen CY, Goossens E. Review of injection techniques for spermatogonial stem cell transplantation. Hum Reprod Update. 2020 Apr 15;26(3):368-391.
Nikiforov, D., Junping, C., Cadenas, J., Shukla, Vallari, Blanshard, R., Pors, S. E., Kristensen, S. G., Macklon, K. T., Colmorn, L., Ernst, E., Bay-Bjørn, A., Ghezelayagh, Z., Wakimoto, Y., Grøndahl, M. L., Hoffmann, Eva, Andersen CY,Improving the maturation rate of human oocytes collected ex vivo during the cryopreservation of ovarian tissue
Journal of Assisted Reproduction and Genetics, volume 37, pages 891–904 (2020)
Albers E, Avram A, Sbroggio M, Fernandez-Capetillo O,Lopez-Contreras AJ Supraphysiological protection from replication stress does not extend mammalian lifespan. Aging (Albany NY). 2020 Apr 6;12(7):5612-5624
Xing M, Zhang F, Liao H, Chen S, Che L, Wang X, Bao Z, Ji F, Chen G, Zhang H, Li W, Chen Z, Liu Y, Hickson ID, Shen H, Ying S.Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged Chromosomes. Mol Cell. 2020 Apr 22. pii: S1097-2765(20)30231-8.
Sankar A, Lerdrup M, Manaf A, Johansen JV, Gonzalez JM, Borup R, Blanshard R, Klungland A, Hansen K, Andersen CY, Dahl JA, Helin K,Hoffmann ER.KDM4A regulates the maternal-to-zygotic transition by protecting broad H3K4me3 domains from H3K9me3 invasion in oocytes. .Nat Cell Biol. 2020 Apr;22(4):380-388
Venegas AB, Natsume T, Kanemaki M, Hickson ID.Inducible Degradation of the Human SMC5/6 Complex Reveals an Essential Role Only during Interphase.Cell Rep. 2020 Apr 21;31(3):107533.
Bizard AH, Hickson ID.The many lives of type IA topoisomerases.J Biol Chem. 2020 Apr 10. Review.
Atashpaz S, Samadi Shams S, Gonzalez JM, Sebestyén E, Arghavanifard N, Gnocchi A, Albers E, Minardi S, Faga G, Soffientini P, Allievi E, Cancila V, Bachi A, Fernández-Capetillo Ó, Tripodo C, Ferrari F, López-Contreras AJ, Costanzo V. ATR expands embryonic stem cell fate potential in response to replication stress. Elife. 2020 Mar 12;9. pii: e54756.
Girardi L, Serdarogullari M, Patassini C, Poli M, Fabiani M, Caroselli S, Coban O, Findikli N, Boynukalin FK, Bahceci M, Chopra R, Canipari R, Cimadomo D, Rienzi L, Ubaldi F, Hoffmann E, Rubio C, Simon C, Capalbo A. I ncidence, Origi n, and Predictive Model f or the Detection and Clinical Management of Segmental Aneuploidies in Human Embry os. Am J Hum Genet. 2020 Mar 19.
Nikiforov D, Junping C, Cadenas J, Shukla V, Blanshard R, Pors SE, Kristensen SG, Macklon KT, Colmorn L, Ernst E, Bay-Bjørn AM, Ghezelayagh Z, Wakimoto Y, Grøndahl ML, Hoffmann E, Andersen CY. Improving the maturation rate of human oocytes collected ex vivo during the cryopreservation of ovarian tissue. J Assist Reprod Genet. 2020 Feb 24.
Ercilla A, Benada J, Amitash S, Zonderland G, Baldi G, Somyajit K, Ochs F, Costanzo V, Lukas J, Toledo L. Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication. Cell Rep. 2020 Feb 18;30(7):2416-2429.e7

Publications 2019

Hildorf S, Cortes D, Gül M, Dong L, Kristensen SG, Jensen CFS, Clasen-Linde E, Fedder J, Andersen CY, Hoffmann ER, Sønksen J, Fossum M, Thorup J. Parental Acceptance Rate of Testicular Tissue Cryopreservation in Danish Boys with Cryptorchidism. Sex Dev. 2019;13(5-6):246-257
Achuthankutty D, Thakur RS, Haahr P, Hoffmann S, Drainas AP, Bizard AH, Weischenfeldt J, Hickson ID, Mailand N.Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability. Journal of Cell Biology 2019 Dec 2;218(12):3943-3953.
Sonneville R, Bhowmick R, Hoffmann S, Mailand N,* Hickson ID*, Labib K*. TRAIP drives replisome disassembly and mitotic DNA repair synthesis at sites of incomplete DNA replication. eLife. 2019 Sep 23;8. e48686. *Co-corresponding author.
Zhang BN, Bueno Venegas A, Hickson ID, Chu WK. DNA replication stress and its impact on chromosome segregation and tumorigenesis. Seminars in Cancer Biology . 2019 Apr; 55:61-69
Taylor AMR, Rothblum-Oviatt C, Ellis NA, Hickson ID, Meyer S, Crawford TO, Smogorzewska A, Pietrucha B, Weemaes C, Stewart GS. Chromosome instability syndromes. Nature Reviews Disease Primers . 2019 Sep 19;5(1):64.
Thompson DJ, Genovese G, Halvardson J, Ulirsch JC, Wright DJ, Terao C, Davidsson OB, Day FR, Sulem P, Jiang Y, Danielsson M, Davies H, Dennis J, Dunlop MG, Easton DF, Fisher VA, Zink F, Houlston RS, Ingelsson M, Kar S, Kerrison ND, Kinnersley B, Kristjansson RP, Law PJ, Li R, Loveday C, Mattisson J, McCarroll SA, Murakami Y, Murray A, Olszewski P, Rychlicka-Buniowska E, Scott RA, Thorsteinsdottir U, Tomlinson I, Moghadam BT, Turnbull C, Wareham NJ, Gudbjartsson DF; International Lung Cancer Consortium (INTEGRAL-ILCCO); Breast Cancer Association Consortium; Consortium of Investigators of Modifiers of BRCA1/2; Endometrial Cancer Association Consortium; Ovarian Cancer Association Consortium; Prostate Cancer Association Group to Investigate Cancer Associated Alterations in the Genome (PRACTICAL) Consortium; Kidney Cancer GWAS Meta-Analysis Project; eQTLGen Consortium; Biobank-based Integrative Omics Study (BIOS) Consortium; 23andMe Research Team, Kamatani Y, Hoffmann ER, Jackson SP, Stefansson K, Auton A, Ong KK, Machiela MJ, Loh PR, Dumanski JP, Chanock SJ, Forsberg LA, Perry JRB. Genetic predisposition to mosaic Y chromosome loss in blood. Nature. 2019 Nov 20.
Abildgaard AB, Stein A, Nielsen SV, Schultz-Knudsen K, Papaleo E, Shrikhande A, Hoffmann ER, Bernstein I, Gerdes AM, Takahashi M, Ishioka C, Lindorff-Larsen K, Hartmann-Petersen R. Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome. eLife. 2019 Nov 7;8. pii: e49138.
Dong L, Kristensen SG, Hildorf S, Gul M, Clasen-Linde E, Fedder J, Hoffmann ER, Cortes D, Thorup J, Andersen CY.
Propagation of Spermatogonial Stem Cell-Like Cells From Infant Boys. Front Physiol. 2019 Sep 19;10:1155.
Zielinska AP, Bellou E, Sharma N, Frombach AS, Seres B, Gruhn JR, Blayney M, Eckel H, Moltrecht R, Elder K, Hoffmann ER, Schuh M Meiotic Kinetochores Fragment into Multiple Lobes upon Cohesin Loss in Aging Eggs Current Biology 29, 1–17.e1–e7, November 18, 2019
Gruhn JR, Zielinska AP, Shukla V, Blanshard R, Capalbo A, Cimadomo D, Nikiforov D, Chan AC, Newnham LJ, Vogel I, Scarica C, Krapchev M, Taylor D, Kristensen SG, Cheng J, Ernst E, Bjørn AB, Colmorn LB, Blayney M, Elder K, Liss J, Hartshorne G, Grøndahl ML, Rienzi L, Ubaldi F, McCoy R, Lukaszuk K, Andersen CY, Schuh M, Hoffmann ER. Chromosome errors in human eggs shape natural fertility over reproductive life span. Science. 2019 Sep 27;365(6460):1466-1469.
Jørgensen SW, Liberti SE, Larsen NB, Lisby M, Mankouri HW, Hickson ID. Esc2 promotes telomere stability in response to DNA replication stress. Nucleic Acids Res. 2019 May 21;47(9):4597-4611
Pladevall-Morera D, Munk S, Ingham A, Garribba L, Albers E, Liu Y, Olsen JV, Lopez-Contreras AJ. Proteomic characterization of chromosomal common fragile site (CFS)-associated proteins uncovers ATRX as a regulator of CFS stability. Nucleic Acids Res. 2019 Sep 5;47(15):8004–8018
Vogel I, Blanshard RC, Hoffmann ER. SureTypeSC - A Random Forest and Gaussian Mixture predictor of high confidence genotypes in single cell data. Bioinformatics. 2019 May 22. pii: btz412. doi: 10.1093/bioinformatics/btz412. [Epub ahead of print]
Borgermann N, Ackermann L, Schwertman P, Hendriks IA, Thijssen K, Liu JC, Lans H, Nielsen ML, Mailand N. SUMOylation promotes protective responses to DNA-protein crosslinks. EMBO J. 2019 Apr 15;38(8). pii: e101496
Bhowmick R, Thakur RS, Venegas AB, Liu Y, Nilsson J, Barisic M, Hickson ID. The RIF1-PP1 Axis Controls Abscission Timing in Human Cells. Current Biology 2019 Apr 1;29(7):1232-1242.e5
Bizard AH, Allemand JF, Hassenkam T, Paramasivam M, Sarlós K, Singh MI, Hickson ID. PICH and TOP3A cooperate to induce positive DNA supercoiling. Nature Structural Molecular Biology. 2019 Apr;26(4):267-274.
Bantele, S.C.S., Lisby, M., and Pfander, B. (2019). Quantitative sensing and signalling of single stranded DNA during the DNA damage response, Nature Communications, 10(1):944.
Klein*, H.L., Bačinskaja, G., Che, J., Cheblal, A., Elango, R., Epshtein, A., Fitzgerald, D.M., Gómez-González, B., Khan, S.R., Kumar, S., Leland, B.A., Marie, L., Mei, Q., Miné-Hattab, J., Piotrowska, A., Polleys, E.J., Putnam, C.D., Radchenko, E.A., Saada, A.A., Sakofsky, C.J., Shim, E.Y., Stracy, M., Xia, J., Yan, Z., Yin, Y., Aguilera, A., Argueso, J.L., Freudenreich, C.H., Gasser, S.M., Gordenin, D.A., Haber, J.E., Ira, G., Jinks-Robertson, S., King, M.C., Kolodner, R.D., Kuzminov, A., Lambert, S.A.E., Lee, S.E., Miller, K.M., Mirkin, S.M., Petes, T.D., Rosenberg, S.M., Rothstein, R., Symington, L.S., Zawadzki, P., Kim*, N., Lisby*, M., and Malkova*, A. (2019). Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways, Microbial Cell, Jan 7;6(1):1-64. *Co-corresponding authors

Publications 2018

Bjerregaard VA, Garribba L, McMurray CT, Hickson ID, Liu Y. Folate deficiency drives mitotic missegregation of the human FRAXA locus. Proc Natl Acad Sci U S A. 2018 Dec 18;115(51):13003-13008

Wild T, Budzowska M, Hellmuth S, Eibes S, Karemore G, Barisic M, Stemmann O, Choudhary C. Deletion of APC7 or APC16 Allows Proliferation of Human Cells without the Spindle Assembly Checkpoint. Cell Reports. 25, 2317–2328, Nov 2018.
Albers E, Sbroggiò M, Pladevall-Morera D, Bizard AH, Avram A, Gonzalez P, Martin-Gonzalez J, Hickson ID, Lopez-Contreras AJ. (2018) Loss of PICH Results in Chromosomal Instability, p53 Activation, and Embryonic Lethality. Cell Rep. 24(12):3274-3284
Sarlós, K., Biebricher, A.S., Bizard, A.H., Bakx, J.A.M., Ferrete-Bonastre, A.G., Modesti, M., Paramasivam, M., Yao, Q., Peterman, E.J.G., Wuite, G.J.L. and Hickson, I.D. (2018) Reconstitution of anaphase DNA bridge recognition and disjunction in vitro. Nature Structural and Molecular Biology 25, 868-876.
Martin CA, Sarlós K, Logan CV, Thakur RS, Parry DA, Bizard AH, Leitch A, Cleal L, Ali NS, Al-Owain MA, Allen W, Altmüller J, Aza-Carmona M, Barakat BAY, Barraza-García J, Begtrup A, Bogliolo M, Cho MT, Cruz-Rojo J, Dhahrabi HAM, Elcioglu NH; GOSgene, Gorman GS, Jobling R, Kesterton I, Kishita Y, Kohda M, Le Quesne Stabej P, Malallah AJ, Nürnberg P, Ohtake A, Okazaki Y, Pujol R, Ramirez MJ, Revah-Politi A, Shimura M, Stevens P, Taylor RW, Turner L, Williams H, Wilson C, Yigit G, Zahavich L, Alkuraya FS, Surralles J, Iglesais A, Murayama K, Wollnik B, Dattani M, Heath KE, Hickson ID, Jackson AP. Mutations in TOP3A Cause a Bloom Syndrome-like Disorder. Am J Hum Genet. 2018 Aug 2;103(2):221-231
Liss J, Pastuszek E, Pukszta S, Hoffmann E, Kuczynski W, Lukaszuk A, Lukaszuk K.
Effect of next-generation sequencing in preimplantation genetic testing on live birth ratio.
Reprod Fertil Dev. 2018 Jun 22. [Epub ahead of print]
McCoy RC, Newnham LJ, Ottolini CS, Hoffmann ER, Chatzimeletiou K, Cornejo OE, Zhan Q, Zaninovic N, Rosenwaks Z, Petrov DA, Demko ZP, Sigurjonsson S, Handyside AH.Tripolar chromosome segregation drives the association between maternal genotype at variants spanning PLK4 and aneuploidy in human preimplantation embryos. Hum Mol Genet. 2018 Jul 15;27(14):2573-2585
Haahr P, Borgermann N, Guo X, Typas D, Achuthankutty D, Hoffmann S, Shearer R, Sixma TK, Mailand N. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability. Mol Cell. 2018 Apr 5;70(1):165-174.e6.
Özer Ö, Bhowmick R, Liu Y, Hickson ID. Human cancer cells utilize mitotic DNA synthesis to resist replication stress at telomeres regardless of their telomere maintenance mechanism. Oncotarget. 2018 Mar 23;9(22):15836-15846.
Bizard AH, Hickson ID. Anaphase: a fortune-teller of genomic instability. Curr Opin Cell Biol. 2018, 8;52:112-119. Review.
Haahr P, Borgermann N, Guo X, Typas D, Achuthankutty D, Hoffmann S, Shearer R, Sixma TK, Mailand N. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability. Mol Cell. 2018 Apr 5;70(1):165-174.e6
Gupta R, Somyajit K, Narita T, Maskey E, Stanlie A, Kremer M, Typas D, Lammers M, Mailand N, Nussenzweig A, Lukas J, Choudhary C. DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity. Cell. 2018 Apr 7. pii: S0092-8674(18)30382-9
Garribba L, Wu W, Özer Ö, Bhowmick R, Hickson ID, Liu Y. Inducing and Detecting Mitotic DNA Synthesis at Difficult-to-Replicate Loci. Methods Enzymol. 2018;601:45-58.
Quevedo O, Lisby M. Imaging of DNA Ultrafine Bridges in Budding Yeast. Methods Mol Biol. 2018;1672:483-493.
Pentzold C, Shah SA, Hansen NR, Le Tallec B, Seguin-Orlando A, Debatisse M, Lisby M, Oestergaard VH. FANCD2 binding identifies conserved fragile sites at large transcribed genes in avian cells. Nucleic Acids Res. 2018 Feb 16;46(3):1280-1294.
Larsen NB, Hickson ID, Mankouri HW. A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation. Methods Mol Biol. 2018;1672:295-309.

Publications 2017

Sarlós K, Biebricher A, Petermann EJG, Wuite GJL, Hickson ID. Knotty Problems during Mitosis: Mechanistic Insight into the Processing of Ultrafine DNA Bridges in Anaphase. Cold Spring Harb Symp Quant Biol. 2017 Nov 22.
Pitchai GP, Kaulich M, Bizard AH, Mesa P, Yao Q, Sarlos K, Streicher WW, Nigg EA, Montoya G, Hickson ID.A novel TPR-BEN domain interaction mediates PICH-BEND3 association. Nucleic Acids Res. 2017 Nov 2;45(19):11413-11424.
Bhowmick R, Hickson ID.The "enemies within": regions of the genome that are inherently difficult to replicate. F1000Res. 2017 May 12;6:666. Review.
Munk S, Sigurðsson JO, Xiao Z, Batth TS, Franciosa G, von Stechow L, Lopez-Contreras AJ, Vertegaal ACO, Olsen JV. Proteomics Reveals Global Regulation of Protein SUMOylation by ATM and ATR Kinases during Replication Stress. Cell Rep. 2017 Oct 10;21(2):546-558.
Bjerregaard VA, Özer Ö, Hickson ID, Liu Y. The Detection and Analysis of Chromosome Fragile Sites.
Methods Mol Biol. 2018;1672:471-482.
Bizard AH, Nielsen CF, Hickson ID. Detection of Ultrafine Anaphase Bridges.
Methods Mol Biol. 2018;1672:495-508.
Capalbo A, Hoffmann ER, Cimadomo d, Ubaldi FM, Rienzi L. Human female meiosis revised: new insights into the mechanisms of chromosome segregation and aneuploidies from advanced genomics and time-lapse imaging
Human Reproduction Update, pp. 1–17, 2017. Review
Larsen NB, Liberti SE, Vogel I, Jørgensen SW, Hickson ID, Mankouri HW.Stalled replication forks generate a distinct mutational signature in yeast. Proc Natl Acad Sci U S A. 2017 Sep 5;114(36):9665-9670
Di Marco S, Hasanova Z, Kanagaraj R, Chappidi N, Altmannova V, Menon S, Sedlackova H, Langhoff J, Surendranath K, Hühn D, Bhowmick R, Marini V, Ferrari S, Hickson ID, Krejci L, Janscak P. RECQ5 Helicase Cooperates with MUS81 Endonuclease in Processing Stalled Replication Forks at Common Fragile Sites during Mitosis. Mol Cell. 2017 Jun 1;66(5):658-671.e8.
Toledo L, Neelsen KJ, Lukas J. Replication Catastrophe: When a Checkpoint Fails because of Exhaustion. Mol Cell. 2017 Jun 15;66(6):735-749. Review.
Natsume T, Nishimura K, Minocherhomji S, Bhowmick R, Hickson ID, Kanemaki MT. Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. 2017 Apr 15;31(8):816-829.
Ren L, Chen L, Wu W, Garribba L, Tian H, Liu Z, Vogel I, Li C, Hickson ID, Liu Y. Potential biomarkers of DNA replication stress in cancer. Oncotarget. 2017 Apr 7.
Kessler BM, Bursomanno S, McGouran JF, Hickson ID, Liu Y. Biochemical and Mass Spectrometry-Based Approaches to Profile SUMOylation in Human Cells. Methods Mol Biol. 2017;1491:131-144.

Publications 2016

Figueira I, Fernandes A, Mladenovic Djordjevic A, Lopez-Contreras A, Henriques CM, Selman C, Ferreiro E, Gonos ES, Trejo JL, Misra J, Rasmussen LJ, Xapelli S, Ellam T, Bellantuono I. Interventions for age-related diseases: Shifting the paradigm. Mech Ageing Dev. 2016 Dec;160:69-92. Review.
Bhowmick R, Minocherhomji S, Hickson ID. RAD52 Facilitates Mitotic DNA Synthesis Following Replication Stress. Mol Cell. 2016 Dec 15;64(6):1117-1126.
Nielsen CF, Hickson ID. PICH promotes mitotic chromosome segregation: Identification of a novel role in rDNA disjunction. Cell Cycle. 2016 Oct 17;15(20):2704-11
Ghamrasni SE, Cardoso R, Li L, Guturi KK, Bjerregaard VA, Liu Y, Venkatesan S, Hande MP, Henderson JT, Sanchez O, Hickson ID, Hakem A, Hakem R. Rad54 and Mus81 cooperation promotes DNA damage repair and restrains chromosome missegregation. Oncogene. 2016 Sep 15;35(37):4836-45
Nieto-Soler M, Morgado-Palacin I, Lafarga V, Lecona E, Murga M, Callen E, Azorin D, Alonso J, Lopez-Contreras AJ, Nussenzweig A, Fernandez-Capetillo O. Efficacy of ATR inhibitors as single agents in Ewing sarcoma. Oncotarget. 2016, 13, 58759-58767
Pitchai GP, Hickson ID, Streicher W, Montoya G, Mesa P. Characterization of the NTPR and BD1 interacting domains of the human PICH-BEND3 complex. Acta Crystallogr F Struct Biol Commun. 2016 Aug;72(Pt 8):646-51
Ottolini CS, Capalbo A, Newnham L, Cimadomo D, Natesan SA, Hoffmann ER, Ubaldi FM, Rienzi L, Handyside AH. Generation of meiomaps of genome-wide recombination and chromosome segregation in human oocytes. Nat Protoc. 2016 Jul;11(7):1229-43
Schwertman P, Bekker-Jensen S, Mailand N. (2016) Regulation of DNA double-strand break repair by ubiquitin and ubiquitin-like modifiers. Nat Rev Mol Cell Biol. 2016 May 23;17(6):379-94.
Lecona E, Rodriguez-Acebes S, Specks J, Lopez-Contreras AJ, Ruppen I, Murga M, Muñoz J, Mendez J, Fernandez-Capetillo O. USP7 is a SUMO deubiquitinase essential for DNA replication. Nat Struct Mol Biol. 2016 Apr;23(4):270-7.
Silva S, Altmannova V, Luke-Glaser S, Henriksen P, Gallina I, Yang X, Choudhary C, Luke B, Krejci L, Lisby M. (2016) Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance. Genes Dev. 2016 Mar 15;30(6):700-17
Ahuja AK, Jodkowska K, Teloni F, Bizard AH, Zellweger R, Herrador R, Ortega S, Hickson ID, Altmeyer M, Mendez J, Lopes M. A short G1 phase imposes constitutive replication stress and fork remodelling in mouse embryonic stem cells. Nat Commun. 2016 Feb 15;7:10660
Haahr, P., Hoffmann, S., Tollenaere, M., Ho, T., Toledo, L., Mann, M., Bekker-Jensen, S., Raschle, M. and Mailand, N. (2016). Activation of the ATR kinase by the RPA-binding protein ETAA1. Nature Cell Biology 18, 1196-1207
Herrtwich L, Nanda I, Evangelou K, Nikolova T, Horn V, Sagar, Erny D, Stefanowski J, Rogell L, Klein C, Gharun K, Follo M, Seidl M, Kremer B, Münke N, Senges J, Fliegauf M, Aschman T, Pfeifer D, Sarrazin S, Sieweke MH, Wagner D, Dierks C, Haaf T, Ness T, Zaiss MM, Voll RE, Deshmukh SD, Prinz M, Goldmann T, Hölscher C, Hauser AE, Lopez-Contreras AJ, Grün D, Gorgoulis V, Diefenbach A, Henneke P, Triantafyllopoulou A. (2016) DNA Damage Signaling Instructs Polyploid Macrophage Fate in Granulomas. Cell 167, 1264-1280
Christensen, C.L., Wulff Helge, J., Krasnik, A., Kriegbaum, M., Rasmussen L.J., Hickson, I.D., Lilsberg, K.B., Oxlund, B., Lau, S.R., Olsen, M.N., Andersen, J.S., Heltberg, A.S., Kuhlman, A.B., Morville, T.H., Dohlmann, T.L., Larsen, S. and Dela, F. (2016) LIFESTAT – living with statins: an interdisciplinary project on the use of statins as a cholesterol-lowering treatment and for cardiovascular risk reduction. Scandinavian Journal of Public Health 44, 534-539
Kessler, B.M., Bursomanno, S., McGouran, J., Hickson, I.D. and Liu, Y. (2016) Biochemical and mass spectrometry-based approaches to profiling SUMOylation in human cells. In Activity-based proteomics (Eds Overkleeft, H. and Florea, B.I.) Methods in Molecular Biology 1491, 131-144

Publications 2015

Kliszczak M, Sedlackova H, Pitchai GP, Streicher WW, Krejci L, Hickson ID. Interaction of RECQ4 and MCM10 is important for efficient DNA replication origin firing in human cells. Oncotarget 6. 2015 Nov 17, 40464-40479

Thorslund T, Ripplinger A, Hoffmann S, Wild T, Uckelmann M, Villumsen B, Narita T, Sixma TK, Choudhary C, Bekker-Jensen S, Mailand N. Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage. Nature. 2015 Nov 19;527(7578):389-93
Newman JA, Savitsky P, Allerston CK, Bizard AH, Özer Ö, Sarlós K, Liu Y, Pardon E, Steyaert J, Hickson ID, Gileadi O. Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes.
Nucleic Acids Res. 2015 Apr 21. pii: gkv373.
Lopez-Contreras AJ*, Specks J, Barlow JH, Ambrogio C, Desler C, Vikingsson S, Rodrigo-Perez S, Green H, Rasmussen LJ, Murga M, Nussenzweig A, Fernandez-Capetillo O*. Increased Rrm2 gene dosage reduces fragile site breakage and prolongs survival of ATR mutant mice . Genes and Development 29, 2015 Apr 1. 690-695 *Co-corresponding authors
Bursomanno S, McGouran JF, Kessler BM, Hickson ID, Liu Y. Regulation of SUMO2 Target Proteins by the Proteasome in Human Cells Exposed to Replication Stress. Journal of Proteome Research 14, 1687-1699.
Chu, W.K., Payne, M.J., Beli, P., Hanada, K., Choudhary, C. and Hickson, I.D. (2015) FBH1 influences DNA replication fork stability and homologous recombination through ubiquitylation of RAD51. Nature Communications 6, 5931
Xing, M., Wang, X., Palmai-Pallag, T., Shen H., Helleday, T., Hickson, I.D. and Ying, S. (2015) Acute MUS81 depletion leads to replication fork slowing and a constitutive DNA damage response. Oncotarget 6, 37638-37646.
Minocherhomji, S., Ying, S., Bjerregaard, V.A., Bursomanno, S., Aleliunaite, A., Wu, W., Mankouri, H.W., Shen, H., Liu, Y. and Hickson, I.D. (2015) Replication stress activates DNA repair synthesis in mitosis. Nature 528, 286-290.
Nielsen, C.F., Huttner, D., Bizard, A.H., Hirano, S., Li, T-N., Palmai-Pallag, T., Bjerregaard, V.A., Liu, Y., Nigg, E.A., Wang, L.H-C. and Hickson, I.D. (2015) PICH promotes sister chromatid disjunction in mitosis: evidence of functional co-operation with topoisomerase II. Nature Communications 6, 8962.
Specks, J., Lecona, E., Lopez-Contreras, AJ. and Fernandez-Capetillo, O. (2015) A Single Conserved Residue Mediates Binding of the Ribonucleotide Reductase Catalytic Subunit RRM1 to RRM2 and Is Essential for Mouse Development. Molecular and Cellular Biology 35, 2910-2917.
Lecona, E., Rodriguez-Acebes, S., Specks, J., Lopez-Contreras, A.J., Ruppen, I., Murga, M., Muñoz, J., Mendez, J. and Fernandez-Capetillo, O. (2016) USP7 is a SUMO deubiquitinase essential for DNA replication. Nature Structural & Molecular Biology